By C.E.R. Jones (Eds.)
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Performance Column performance was maintained by monthly servicing, in which the flame ionization detectors and associated inlet connectors were cleaned in order to remove relatively nonvolatile residues. Also, the first 10 cm length of the column packing, sited within the injection zone of the Chromatograph, was replaced by fresh packing. A standard washed dried yeast preparation was used for regular checks on output performance. Peak height differences of 8% were typical for these checks. 60 Evaluation Less than half the pyrogram peaks showed the degree of variability considered suitable for discriminating between oral streptococci.
Its range of application starts with hydrophilic and ends with lipophilic mixtures of substances , Its greatest advantage - compared with gas chromatography and HPLC - is the possibility of application of hundreds of reagents and a number of different biological and physico-chemical procedures for the detection of separated substances. Chromatography should not be seen as one step in itself, it is essential always to differentiate the various steps: 1) Preparation of samples, e. g. separation from polymer material 30 2) Actual Chromatographie procedure 3) Detection of the mostly colourless separated substances 4) Safe identification of individual components in the μg-range without Rfvalues.
3 min hold, 50°-(4)-180°. Gases, H - 30 ml/min; Air 300 ml/min, He - 2 ml/min make-up, 28 ml/min. Pyroprobes 100: 800° for 10 sec. Ramp - 75°/msec; Interface - 200°. 5 mm, 20M. SO/ig Figure 3. H. P. 6 min hold, 55°- (6)-165°. Gases, H 20 ml/min, Air - 240 ml/min, N - 25 ml/min. Pyroprobe: 900° for 10 sec, Ramp - 75°/msec; Interface - 180°. 7m 2 mm glass. 53 organisms responsible for such scourges as botulism and the gas gangrene occuring in war wounds, particularly those obtained in the course of the 1914-1918 war.
Analytical Pyrolysis by C.E.R. Jones (Eds.)