Download PDF by M L Anson: Advances in Protein Chemistry, 6

By M L Anson

ISBN-10: 008058179X

ISBN-13: 9780080581798

ISBN-10: 0120342065

ISBN-13: 9780120342068

ADVANCES IN PROTEIN CHEMISTRY VOL 6.

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Although the general phenomenon of light scattering has been well understood for several decades, it is only within the last few years that its utility in the study of macromolecules has been clearly established. As a consequence it is now possible to determine the size, weight, and activity coefficient of macromolecules in solution by making relatively simple optical measurements. The method is both supplementary and complementary to other physical methods of investigating the properties of macromolecules and therefore offers both a means of measuring constants otherwise obtainable and of providing completely new information.

VI. Experimental Methods. . . . . . . . . . . . . . . . . . . . . . . Although the general phenomenon of light scattering has been well understood for several decades, it is only within the last few years that its utility in the study of macromolecules has been clearly established. As a consequence it is now possible to determine the size, weight, and activity coefficient of macromolecules in solution by making relatively simple optical measurements. The method is both supplementary and complementary to other physical methods of investigating the properties of macromolecules and therefore offers both a means of measuring constants otherwise obtainable and of providing completely new information.

2. Turbidity by Transmission Measurements. . . . . . . . . . . . . . 108 3. Measurements of Reduced Intensity of Scattering.. . . . . . . . . . 109 4. ions. . . . . . . . . 111 5. The Use of Working Standards. . . . . . . . . . . . . . . . . . . 113 6 . Depolarisation Measurements. . . . . . . . . . . . . . . . . . . 114 7. Specific Refractive Index Increments. . . . . . . . . . . . .

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Advances in Protein Chemistry, 6 by M L Anson


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